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osubip cas9 nost  (Addgene inc)


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    Structured Review

    Addgene inc osubip cas9 nost
    Osubip Cas9 Nost, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pbi+mcs+egfp/pBI-MCS-EGFP+(Plasmid+%2316542)/pm41662274-60-44-45
    Average 93 stars, based on 24 article reviews
    osubip cas9 nost - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Functional genetic variants can mediate their regulatory effects through alteration of transcription factor binding
    Article Snippet: To generate the dCas9 vectors, plasmid pAC154-dual-dCas9VP160-sgExpression (Addgene plasmid # 48240) was double digested, then purified on agarose gel to obtain a linearized fragment containing both the sgRNA expression and the dCas9 sequences. .. P2A Reverse complement oligonucleotides were annealed and extended, while the EGFP sequence was amplified by PCR from plasmid pBI-MCS-EGFP (Addgene plasmid #16542) using the nucleotides and primers described in Supplementary Data . ..

    Article Title: A cellular stress response induced by the CRISPR/dCas9 activation system is not heritable through cell divisions
    Article Snippet: .. The GFP sequence was amplified by PCR from plasmid pBI-MCS-EGFP (Addgene plasmid #16542) and all fragments were Gibson assembled to provide the sgRNA-dCas9-VP160-2A-GFP vector. .. Additional steps of plasmid digestion, gel purification, and Gibson assembly were then applied to the resulting vector.

    Amplification:

    Article Title: Functional genetic variants can mediate their regulatory effects through alteration of transcription factor binding
    Article Snippet: To generate the dCas9 vectors, plasmid pAC154-dual-dCas9VP160-sgExpression (Addgene plasmid # 48240) was double digested, then purified on agarose gel to obtain a linearized fragment containing both the sgRNA expression and the dCas9 sequences. .. P2A Reverse complement oligonucleotides were annealed and extended, while the EGFP sequence was amplified by PCR from plasmid pBI-MCS-EGFP (Addgene plasmid #16542) using the nucleotides and primers described in Supplementary Data . ..

    Article Title: A cellular stress response induced by the CRISPR/dCas9 activation system is not heritable through cell divisions
    Article Snippet: .. The GFP sequence was amplified by PCR from plasmid pBI-MCS-EGFP (Addgene plasmid #16542) and all fragments were Gibson assembled to provide the sgRNA-dCas9-VP160-2A-GFP vector. .. Additional steps of plasmid digestion, gel purification, and Gibson assembly were then applied to the resulting vector.

    Polymerase Chain Reaction:

    Article Title: Functional genetic variants can mediate their regulatory effects through alteration of transcription factor binding
    Article Snippet: To generate the dCas9 vectors, plasmid pAC154-dual-dCas9VP160-sgExpression (Addgene plasmid # 48240) was double digested, then purified on agarose gel to obtain a linearized fragment containing both the sgRNA expression and the dCas9 sequences. .. P2A Reverse complement oligonucleotides were annealed and extended, while the EGFP sequence was amplified by PCR from plasmid pBI-MCS-EGFP (Addgene plasmid #16542) using the nucleotides and primers described in Supplementary Data . ..

    Article Title: A cellular stress response induced by the CRISPR/dCas9 activation system is not heritable through cell divisions
    Article Snippet: .. The GFP sequence was amplified by PCR from plasmid pBI-MCS-EGFP (Addgene plasmid #16542) and all fragments were Gibson assembled to provide the sgRNA-dCas9-VP160-2A-GFP vector. .. Additional steps of plasmid digestion, gel purification, and Gibson assembly were then applied to the resulting vector.

    Plasmid Preparation:

    Article Title: Functional genetic variants can mediate their regulatory effects through alteration of transcription factor binding
    Article Snippet: To generate the dCas9 vectors, plasmid pAC154-dual-dCas9VP160-sgExpression (Addgene plasmid # 48240) was double digested, then purified on agarose gel to obtain a linearized fragment containing both the sgRNA expression and the dCas9 sequences. .. P2A Reverse complement oligonucleotides were annealed and extended, while the EGFP sequence was amplified by PCR from plasmid pBI-MCS-EGFP (Addgene plasmid #16542) using the nucleotides and primers described in Supplementary Data . ..

    Article Title: A cellular stress response induced by the CRISPR/dCas9 activation system is not heritable through cell divisions
    Article Snippet: .. The GFP sequence was amplified by PCR from plasmid pBI-MCS-EGFP (Addgene plasmid #16542) and all fragments were Gibson assembled to provide the sgRNA-dCas9-VP160-2A-GFP vector. .. Additional steps of plasmid digestion, gel purification, and Gibson assembly were then applied to the resulting vector.



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